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Dead cells were detected with a ReadyProbes Cell Viability Imaging Kit Blue/Green (Invitrogen): after the end of experiments, two drops per ml of NucBlue Live and NucGreen Dead reagents were added into each well and incubated for 15/30 min at RT to stain the nuclei of all cells (blue) and detect dead cells (green), respectively

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However, it may not be the best choice for individuals with more complex needs who require ongoing oversight