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The immune system becomes hyper-vigilant and metabolically demanding, hoarding the body's limited ATP reserves

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10 mg vial + 3 mL bacteriostatic water 10 mg 3 mL = 3.33 mg/mL At this concentration the same 0.25 mg starting dose becomes 0.25 3.33 = 0.075 mL 7.5 units , and the 2.4 mg maintenance dose becomes 2.4 3.33 = 0.72 mL = 72 units

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p53 TAD (13-61) was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3) pLysS cells

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