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These may include poor sleep, low protein intake, under-eating during weight loss, blood sugar swings, anemia, thyroid dysfunction, menopause or testosterone changes, chronic stress, inflammation, medication side effects, poor conditioning, depression, sleep apnea, liver concerns, kidney issues, or nutrient deficiencies

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After that, 1 ml diethyl ether was added to the samples to extract SCFAs, then samples were centrifuged at 14,000 g , 4 C, 15 min, and the supernatant was used for GC-MS 55