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Isolated CD4 + and CD8 + T cells were activated or not for up to 1, 3, or 6 days in a reverse time course with Dynabeads (1:1 cell-to-bead ratio, Gibco), magnetic beads similar in size to antigen-presenting cells and coupled with anti-CD3 and anti-CD28 antibodies, so that their total time in culture would be identical and to ensure that all cells would finish their indicated activation period the same day

Homeostatic model assessment, plasma and erythrocyte Se concentration, GPX activity, SELENOP concentration, and other Se biomarkers will be considered secondary outcomes

When compared with the respective CON-fed groups, EtOH dramatically induced hepatic protein carbonylation in both WT and KO mice, albeit the levels in the KO mice remained higher than those observed in WT mice

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