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Cholesterol modification treatments To increase cholesterol, islets isolated from chow-fed C57BL/6 WT mice were pre-incubated in 20 mM cholesterol loaded onto methyl--cyclodextrin (MCD) (C4951, Sigma Aldrich) diluted in 1X Krebs-Ringer bicarbonate-Hepes (KRBH) buffer [140 mM NaCl, 3.6 mM KCl, 1.5 mM CaCl 2 , 0.5 mM MgSO 4 , 0.5 mM NaH 2 PO 4 , 2 mM NaHCO 3 , 10 mM Hepes, saturated with 95% O 2 / 5% CO 2
doi: 10.3389/fmed.2021.649408
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Iran J Public Health
Once released into the circulation, GLP-1 binds to the GLP-1 receptor, which is expressed in the pancreas, gastrointestinal tract, kidney, heart and brain
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