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Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates

JAMA 2007;298:2038e47

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Experiencing these symptoms can be alarming, especially when they happen suddenly or persist over time

The CU suffix denotes its association with a copper (II) ion, which the peptide binds with high affinity through the imidazole group of histidine and the amino-terminal amine