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In an unmatched population-based casecontrol approach, we estimated a sample size of 258 individuals with QUANTO software, version 1.2.4, assuming a log-additive inheritance model at 5% significance and 80% power and using the following parameters based on our previous study 20 : an environmental exposure to Hg of 2.5%, a population risk of reduced estimated glomerular filtration rate (eGFR