LC-MS confirms identity by measuring the molecular mass and comparing it to the theoretical value (403404 g/mol for the parent complex, 462 g/mol for the acetate salt)
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To validate the interaction at the nuclear envelope, immunofluorescence studies were performed, in infected cells by labelling HA (for the HSV-1 U L 31 -HA virus) and pU L 34 (as a NEC and nuclear membrane marker)
2) A blood draw was conducted to test for blood chemistry (including kidney and liver function) and Complete Blood Count (CBC), and Red Blood Cell (RBC) glutathione