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For concomitant negative selection against an off-target substrate, an orthogonal autoinhibited T7 variant is encoded with the off-target substrate such that off-target cleavage activates transcription of gIII-neg, a dominant negative variant that suppresses phage propagation 6

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1 HCV is classified into six different genotypes (numbered 1-6), with each genotype divided into subtypes

Mller Foundation, the Novo Nordisk Foundation, the Danish Medical Association research grant, the Australian Research Council, the National Health and Medical Research Council, and Pfizer Inc

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