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coli BL21(DE3) cys cells cotransformed with plasmids pCGPx3M and pMazF (TaKaRa) were grown at 37C in 400 mL of MM medium (modified M9 minimal medium) supplemented with ampicillin (100 g/mL), kanamycin (25 g/mL), chloramphenicol (25 g/mL) and Cys (50 g/mL) on a shaker
Furthermore, PS1V97L transgenic mice exhibited increased inflammatory cytokines including IL-1 and TNF, which were attenuated by sulforaphane supplementation (Hou et al., 2018)
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