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However, in agreement with our detected concentrations by HF-LPME, previous measured whole-body concentrations of weakly acidic sulfonamides and weakly basic diphenhydramine in fish significantly increased with increases in the neutral molecule forms 31,32 , suggesting that the acceptor concentration in HF-LPME can be used as a surrogate for the internal concentration in D

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However, the MBC exhibited obvious degradation behavior with disruption of the membrane structure with the time prolonging to 7 days (Supplementary Fig

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