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Next, cells were centrifuged and washed twice with FACS buffer (1x PBS, 0.05% Bovine Serum Albumin, 0.05% NaN 3 ), resuspended in FACS buffer, and analyzed using either a FACSCalibur machine (BD Biosciences) or a CytoFlex machine (Beckman-Coulter Life Sciences) and the FCS Express software (De Novo Software)

GLOW contains multiple peptides designed to investigate broader recovery pathways, while BPC-157 is a single peptide often focused on connective tissue and gastrointestinal research

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The methylcobalamin form you were given can seem to cause anxiety or a wired feeling in some people, due to the way their body processes the methyl part

Site- and condition-specific imputation was performed using the scImpute function in PhosR ( An FDR threshold of 0.1 was implemented