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The peptides under investigation, include: Nle-AngIV (Nle-Tyr-Ile-His-Pro-Phe) and its analogs such as D-Nle-YIH, acetyl-NleYIH, -amino butyric acid-YIH, NleYI-amide, and the primary focus, N-hexanoic-Tyr-Ile-(6) aminohexanoic amide (Dihexa)

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However, ketamine induces a complex response unfolding distinct influences over time, producing antidepressant effects as fast as 30 min after administration at 0.5 mg/kg in humans 6 and at a comparable dosage of 10 mg/kg in rat models 7,8

b) transforming said expression vector into host cell and inducing the expression to obtain expressed semapeptide