The cells (3 10 6 cells/mL) were transfected with 1 g of plasmid DNA per mL of cell culture using ExpiFectamine 293 Reagent (Gibco)
Conflict of interest The authors declare no conflict of interest Authors contributions Conceptualization, formal analysis, original draft preparation (SKC), writingreview and editing (SKC, DC), supervision, project administration, and funding acquisition (SKC)
Limited clinical data exists, but anecdotal reports suggest constipation rates around 1015% at doses above 600mg daily
At the time of discharge, the patient exhibited slightly decreased strength in the left upper extremity with a persistent pronator drift
Much of the stronger -opioid signaling observed in vivo is attributed to metabolic formation of 7-OH, which is far more potent