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Promega) (for AMYRs) or coelenterazine h (Nanolight Technologies, Pinetop, AZ) (for CTR) was then added to cells at a final concentration of 1:1000 or 5 M, respectively, and incubated for a further 1015 min

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The symptoms of such conditions include: Sudden and extreme pain in the upper right or center of the abdomen Fever and jaundice (a yellow tint to the skin or eyes) Nausea and vomiting These issues can lead to significant complications if not treated promptly, requiring medical intervention to manage the pain and remove the blockages

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Most light chain SLC7s interact with SLC3A2, while SLC3A1 forms heterodimers with SLC7A9, which are associated with cystinuria (50)