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DsbASP was oxidized with a 10-fold molar excess of AT-2 and roGFP2 was reduced with a 10-fold molar excess of DTT for 10 min at 20 C and then the oxidant or reductant were removed using Micro Bio-Spin P-30 gel columns (BioRad, Feldkirchen, Germany, #7326223) or NAP-5 columns (Cytiva, Freiburg, Germany, #17085302) according to the suppliers protocol

Finally, cut and purify from the resin

doi: 10.3389/fmolb.2021.649151 Received 04 January 2021 Accepted 12 February 2021 Published 13 April 2021 Volume 8 - 2021 Edited by Graa Soveral, University of Lisbon, Portugal Reviewed by Pdraig D'Arcy, Linkping University, Sweden Benita Sjogren, Purdue University, United States Updates Copyright 2021 Chen, Dou, Liu and Tang

Lee K, Won HY, Bae MA, Hong JH, Hwang ES

Cagrilintide is a synthetic peptide manufactured for scientific research and analytical testing