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Garg VK, Sarkar R, Agarwal R

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Possible damages induced by MSG effects on some organs have been stated in experimental animal models

The blots were probed with the primary antibodies specific for phosphorylated PERK and CHOP (Thermo Fischer Scientific Inc.) at 4C overnight, then the blots were rinsed 35 times for 5 min with TBST and incubated with the horseradish peroxidase (HRP)-conjugated anti-rabbit IgG Secondary Antibody (Thermo Fischer Scientific Inc.) against the blotted target protein for 1 h at room temperature