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Enzyme expression was induced by the addition of 100 M isopropyl -d-1-thiogalactopyranoside, the cultures were incubated for another 3.5 h at 30 C, 140 rpm using a 5 cm shaking diameter, and the final OD 600 (optical density at 600 nm) was determined using a CO8000 cell density meter (WPA biowave)

30 GhiranIGlodekAMWeaverGKlicksteinLBNicholson-WellerA

doi: 10.1016/j.hlc.2022.12.015 Foster, Victoria, Saez, Natalie, Gillespie, Ellen, Jogia, Trisha, Reid, Chantelle, Maljevic, Snezana, Jung, Woncheol, Lao, Hong W., Ruitenberg, Marc Jan and King, Glen (2023)

doi: 10.1016/j.bioadv.2023.213755

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