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On the assay day, the cells were washed two times with binding buffer (50 mM HEPES buffer (pH 7.2), 1 mM CaCl 2 , 5 mM MgCl 2 , 0.5% (w/v) BSA) and incubated with binding buffer for 15 min at 4 C

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Glutathione is a water-soluble active that does not readily penetrate the skin on its own, which is why advanced formulation strategies are essential to enhance its delivery and bioavailability

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