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Given that PKC and TRPM5 are downstream effectors of Gq signalling in beta cells, our next step involved determining whether the PKC inhibitor (calphostin C) and TRPM5 blocker (TPPO) suppress the triagonist-induced increase in GSIS

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The accessibility in the external part of the TM5 of the glutamate transporter EAAT1 is conformationally sensitive during the transport cycle

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