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Stromal vascular fraction isolation For stromal vascular fraction (SVF) isolation, harvested sWAT depots from male C57Bl/6J mice were finely minced and digested in collagenase solution (2 mg/ml collagenase Type II, 10 mM CaCl2 and 0.5% free fatty acid free BSA in PBS) for 30 min at 37 C at 16 g
Their expertise in recognizing patterns of adipose tissue distribution, assessing skin changes, and distinguishing lipedema from other disorders is critical for early and accurate diagnosis
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This created four conditions: Sal + Sal, Sal + Ex-4, Her + Sal, and Her + Ex-4
