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Gene-specific primer pairs used in the study (Supplementary Table 1) were designed or confirmed from sequences established in the literature 6,124,125 to span exon-exon junctions or large introns to avoid possible amplification of genomic DNA

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One milligram kept most of the 2.4 mg weight effect in a diabetes trial, and 0.5 mg has years of use behind it. Small real-world series also suggest that reduced-frequency dosing can hold a plateau