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employed a combination of reversed phase (C19 column) and hydrophilic interaction liquid chromatography (HILIC) chromatography for a better coverage of the metabolome in the non-polar and polar fractions, respectively (Liu et al., 2020) evaluated the sensitivity and reproducibility of an HILIC method, assessed the influence of mobile phase gradient, ionic strength and column temperature and validated the method for glycolysis pathway metabolites upon exposing Huh-7 cells to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (Rodrigues et al., 2018)

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Ibuprofen significantly inhibited IgG production only in wild-type splenocytes (by ~ 70%) but did not inhibit IgG production in Cox-2 knock-out mice (Figure 3B

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