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(1996) shows that when GH and IGF-1 are combined, they can significantly counteract weight loss and promote weight gain, particularly in the case of burned rats

18 h later, the cells in each well were washed with PBS once, then incubated with 1 ml PBS containing 2 M C11-BODIPY 581/591 (Invitrogen) at 37 C for 15 min

This is consistent with NOS block preventing the beneficial effect of RPc on no-reflow in vivo 29