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Then continue with your usual skincare routine according to your skin condition

Journal Reference : Fatima Quddos, Mary Fowler, Ana Carolina de Lima Bovo, Zacarya Elbash, Allison N

Repeated sunburns accelerate skin aging and significantly increase skin cancer risk

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

A substance that regulates cell growth and immune responses, such as inflammation References : Button at al (2018), Roles of mucus adhesion and cohesion in cough clearance, PNAS Flora et al.,(1997), Attenuation of influenza-like symptomatology and improvement of cell-mediated immunity with long-term N-acetylcysteine treatment