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Hess, J., Keiser, J

Immunoblotting To obtain whole-cell extracts for immunoblotting, cells were resuspended with RIPA buffer (50 mM Tris pH 8.0, 250 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.05% SDS, 1 mM DTT) on ice for 20 min, followed by centrifugation at 20,000 g for 20 min

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The expression profile of genes monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor (TNF), tumor necrosis factor receptor type 1 (TNFR1), transforming growth factor-beta-1 (TGF1), collagen type I alpha 1 chain (Col1,) actin alpha 2 smooth muscle (SMA), tissue inhibitor of metalloproteinases 1 (Timp1), and haptoglobin (Hp) associated with non-alcoholic fatty liver disease (NAFLD) were elevated in the livers of mice fed an HFD for 16 weeks compared with those from mice fed the control diet (Fig

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