Viral infection For lentiviral production, HEK293T cells were co-transfected with 4 g 8.91, 1 g pVSVG, 5 g of lentivector (shRNA targeting SHMT2 : CCAGAATTTGTAGCTGAGATT, and ATF4 : GCGAGTGTAAGGAGCTAGAAA, obtained from RNA Technology platform and Gene manipulating core, Academia Sinica) by using 30 L of Turbofect (Thermo, R0533) in 1 mL of Opti-MEM (Gibco, 31985070) according to the manufacturers instructions
(2018) 17:e12699
Nothing here is medical advice, a therapeutic claim, or a dosing recommendation for human use
The exact response depends on your individual genetics, baseline gut bacteria, diet, and hydration status
Materials PEG ( M n = 6000 g mol 1 ), MDI, IU, DBTDL, DMF, DPPH (2,2-diphenyl-1-picrylhydrazyl) and TA were purchased from Aladdin (Shanghai, China)