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Estrogenic activity of two standardized red clover extracts (Menoflavon) intended for large scale use in hormone replacement therapy

Luciferin detection reagent (LDR) (100 l) was added to all wells, briefly shaken and incubated for 15 min at room temperature and the luminescence measured using microplate imager (BioTek Cytation3)

Interestingly, a recent study has even suggested that vitamin supplements may increase the oxidant stress

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The activation index in each condition was evaluated by determining the percentage of bromodeoxyuridine (BrdU)-positive cells, indicative of the cell-activation response to HGF samples