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Urinary sodium and potassium levels were determined using Quantichrom Assay Kits (BioAssay Sytems) and then fractional excretion of sodium (FENa) and fractional excretion of potassium (FEK) were calculated using the corresponding formulas ( TUNEL Assay To assess tubular cell death, TUNEL assay was used on kidney section embedded in paraffin according to the products protocols (Roche, Basel, Switzerland)

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These two individually randomized cross-over studies demonstrated a poor quality assessment bias [19]

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