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Use only the ring finger (least pressure), apply with gentle tapping or pressing along the orbital bone (never on the lid), use 1 drop per eye maximum, avoid direct contact with the eye, allow to absorb before applying any moisturizer, and never rub or drag the periocular skin

Briefly, the cells were cultured in Dulbeccos modified Eagle medium (DMEM, Sigma), supplemented with 10% FBS, and kept in an incubator at 37 C and 5% CO 2

The molecular response to heat includes increased expression of HSPs, transcriptional regulators, such as NRF2, and pro- and anti-inflammatory factors (Patrick and Johnson, 2021)

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185 SIRT1 is essential for controlling ROS production, maintaining mitochondrial function, and regulating selective autophagy