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Similar content being viewed by others Introduction Tumor necrosis factor-alpha (TNF-) is a clinically relevant cytokine that regulates activities such as cell growth, carcinogenesis, inflammation, viral replication, immune surveillance, and autoimmunity 1

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The complex was eluted from the resin and concentrated to 500 L using a 100 kDa MWCO Amicon Ultra Centrifugal Filter (Merck Millipore, Darmstadt, Germany)

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typically resolves within hours [4][6]