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U3085 cells were seeded at a density of between 80,000100,000 cells/mL and allowed to adhere for 48 h prior to treatment with lobeline/vehicle control in serum-free DMEM (does not contain sodium pyruvate [18]) for 1 h at 37 C

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The interaction is facilitated by the microbiota, but a dysbiotic biota can lead to brain inflammation and psychopathologies as depicted in Figure 6 (86, 87)