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Cell extracts were prepared by sonication in bacteria lysis buffer (50 mM Tris, 20% glycerol, 150 mM NaCl, 0.5 mM EDTA, 1 % NP-40, 2 mM PMSF, cOmplete Mini Protease Inhibitor Cocktail (1 tablet per 50 ml, Roche, 04693124001))

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4a).These metabolites are involved in one-carbon metabolism, methylation processes, and amino acid biosynthesis, suggesting coordinated alterations in pathways relevant to cellular methylation and metabolic regulation in ASD

doi: 10.3390/jcm13216292 53 HoganAEGaoatsweGLynchLCorriganMAWoodsCOConnellJet al

12 February 2026