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Immunoblotting The supernatant containing pseudotyped viruses was mixed at a 3:1 (v/v) ratio with Retro-X Concentrator (Cat# 631455, Clontech) and incubated overnight at 4 C

GLP-1 receptors are present in vascular tissue, and their activation may improve endothelial function

2020 Mar;127:154959

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While these bacterial enzymes have been explored for the generation of natural product analogs or applications in biocatalysis, the broad utility of amide bond-forming enzymes in enzymatic combinatorial synthesis has not been fully realized because of unwanted cross-reactivities of the enzymes toward substrates and/or difficulties in finding partnering enzymes with comparable substrate promiscuity 56