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Overnight cultures were transferred to conical flasks containing LB broth and grown to an optical density (OD 600 ) of 0.35

doi: 10.1128/iai.64.9.3703-3712.1996 122 MackenzieG

Whilst they affect gut function by slowing gastric emptying and influencing motility, their gastrointestinal side effectsincluding nausea, diarrhoea, constipation, and bloatingmay overlap with or potentially worsen existing IBS symptoms

If a dog can still smell their previous accident, they will instinctively return to that exact spot to go again

Nocturnal timing becomes more important with age, not less, because youre working with a smaller baseline pulse