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After removing the magnetic beads, samples were spun down at 20,000 g for 10 min to remove the remaining beads, and the supernatant was transferred to a scintillation vial with 5 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339)

Both share the same evidence base and the same cycle length range

Treatment of the embryos with NOx led to a slight increase in GSSG concentration after 7 days of ageing, but the result was not statistically significant

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