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The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow

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There are three enzymes contributing to UPP: E1 (Ub-activating enzyme) and E2s (Ub-carrier) prepare Ub for conjugation, but the main enzyme in the process is the E3 (Ub-protein ligase) which transfers the activated ubiquitin to the protein substrate to be degraded [66]

Antioxidante maestro: Ms poderoso que la vitamina C o E, el glutatin protege las clulas del dao oxidativo y neutraliza los radicales libres que causan el envejecimiento