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Imaging and colony counting were performed using Quantity One 1-D analysis software (Bio-Rad, Hercules, CA, USA)

The mechanism is still unclear, but one oft-cited study of women who were overweight or obese and also had prediabetes found that NAD supplementation with nicotinamide mononucleotide (NM), an NAD precursor, for 10 weeks improved insulin sensitivity

98 Although berberine has been studied in human clinical trials and shown to have several metabolic benefits, concerns about long-term use of berberine have been raised on the basis of certain preclinical studies

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Each value was presented as the mean standard deviation (SD), with a minimum of three replicates