Other peptides under investigation Several additional peptides are being studied for disc-related applications, though with less established evidence: Vasoactive Intestinal Peptide (VIP) has demonstrated the ability to slow disc degeneration progression and improve aggrecan levels in mouse studies
You spent the dose and got a fraction of the response
Cell culture TC1 clone 6 (TC16) cells [American Type Tissue Culture Collection (ATCC), #CRL-2934, Manassas, VA] were cultured in DMEM medium (Gibco) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin, 15 mM HEPES, 0.1 mM non-essential amino acids, 0.02% BSA, 15 mg/L sodium bicarbonate, 2 mg/L glucose
If you add too little, the solution will not be bacteriostatic, and bacteria will grow in the vial
Pulmonary fibroblasts were isolated from the 24-week-old fetus, and it was observed that these fibroblasts appeared to lose their proliferative function at the 34th passage