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Lotte Bjerre Knudsen (32:02): I don't know about health span, but because certainly there's been so many studies with metformin and there's been a lot of wonderful data showing an effect on the epigenetic clocks, but not really an effect on lifespan because that metformin is so widely used

American Heart Association, 2024)

However, compared to the controls, the calorie restriction group had a statistically significant 39% increased risk of major osteoporotic fractures (hazard ratio [HR], 1.39

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Powdered tissue (0.2 g) was homogenized with 0.1% triuoroacetic acid, centrifuged, and the supernatant was collected