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Analytical evaluation commonly includes peptide identity confirmation, purity assessment using validated chromatographic techniques such as high-performance liquid chromatography (HPLC), and batch reproducibility verification

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The proteins were visualized by exposure machine (GE, Amersham Imager 680) using Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific, 32132) and the protein bands were quantitatively analyzed with ImageJ software

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This simple practice dramatically extends effective shelf life