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Cells were collected by scraping and then lysed on ice with a lysis buffer (20 mM HEPES pH 7.4, 142 mM KCl, 5 mM MgCl 2 , 1 mM EDTA, 5% glycerol, 1% NP40 and 0.1% SDS) supplemented with freshly added protease (1/200 dilution, #P8340, Sigma Aldrich) and phosphatase (1/400 dilution, #P5726, Sigma Aldrich) inhibitors

GLP-1 medications are getting a lot of attention right nowand for good reason

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HILTON HEAD, SC Sitting around a relaxed classroom with a handful of other guests at Hilton Head Health Wellness Resort & Spa in South Carolina, I never would have guessed anyone was on or considering a GLP-1 medication

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