The cell viability was assessed by Hoechst staining of the nucleus (see Methods) and hence assessment of total cell numbers after 72 h of treatment, which is a good proxy for cell survival and cell growth upon prolonged compound exposure
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Superoxide Dismutase (SOD), the Youth Molecule, is the only antioxidant agent able to regulate the production of superoxide anion radicals, thus preventing cell oxidation and damage, Superoxide anions are one of the strongest reactive oxygen species (ROS) and contribute to many health challenges, SOD neutralises these radicals into hydrogen peroxide and oxygen, Through aging and poor health, our SOD capacity diminishes, accelerating cellular damage, SOD can help manage cellular damage due to aging or illness
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Negative control reactions were performed with buffer in place of the purified enzymes (neg)