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The stained slides were then observed under an inverted fluorescence microscope (DMi8, Leica, Germany)

Signs and Symptoms of GAD People with GAD may experience: Excessive worry on most days Trouble controlling anxious thoughts Feeling nervous, restless, or on edge Trouble focusing or concentrating Irritability or mood changes Muscle tension Headaches or stomach discomfort Feeling tired often Trouble falling asleep or staying asleep Feeling overwhelmed by small tasks Worrying even when there is little or no clear reason These symptoms can make simple daily activities feel stressful or exhausting

Thus, after transcription and translation, the GST protein and the protein of interest will be expressed together as a fusion protein

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