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This resistance mechanism has been observed in both laboratory-generated resistant strains and clinical isolates, underscoring its relevance in both experimental and field settings (Pountain et al., 2019).The altered sterol profile, often featuring ergosta-7,22-dienol or cholesta-5,7,22-trienol, compromises the drugs efficacy, indicating that mutations in sterol metabolism are central to AmB resistance in Leishmania (Yao and Wilson, 2016