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Next, the sections were incubated with horseradish peroxidase-conjugated anti-rabbit antibodies (Alpha Diagnostic, San Antonio, TX, United States) for 20 min in a 37C incubator and then rinsed with PBS three times for 5 min each

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The behaviors that were measured in this study include: the number of myoclonic jerks, the latency to the myoclonic jerks and tonic-clonic seizures, and also the duration of tonic-clonic seizures

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