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Effect of insulinotropic agent nateglinide on Kv and Ca(2+) channels in pancreatic beta-cell
However, 'forever' may not be accurate for everyone
Lancet 2008, 372: 12401250
For recycling, cells were incubated with agonists at 37 C for 15 min, followed by endocytosis arrest at 4 C, surface label removal as above, 30 min incubation at 37 C with 10 M exendin(9-39) to prevent further internalization, and a second round of surface label removal at 4 C
Three specific conditions qualify for Tier 2 (BMI 30 or higher): (1) heart failure with preserved ejection fraction (HFpEF), (2) uncontrolled hypertension despite treatment with two or more antihypertensive medications (systolic over 140 or diastolic over 90), and (3) chronic kidney disease stage 3a or higher (estimated GFR below 60)
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