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Retrieved from [v] Higenamine: What Athletes Need to Know to Compete Clean: USADA

To verify the absence of bacteria, flies from two different germ-free vials were crushed in ~ 200uL of sterile dH2O in an aseptic environment, then plated on blood agar

This documentation supports experimental reproducibility and enables troubleshooting if unexpected results emerge

RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA1 but not VLA-4 integrin mediated human T-cell adhesion