This removes the water without passing through a liquid phase, which is incredibly gentle on the peptides fragile structure
Curiously, in 1984 it was recognized that the actual reasons for the electron density missing from a protein structure can be very different (Garner et al., 1998)), i.e., the wholesale movement of a structured domain facilitated by a flexible hinge
The location of Cu B is better conserved than that of Cu A , which is in agreement with the previous observations [12,26,31] as well as with the recently elucidated crystal structure of Zn(II)-bound TRP1 [25]
Reitz C, Brayne C, Mayeux R
You might feel a slight pinch at the start, but after that, most people feel completely fine