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Fusion constructs ROGFP2 WT -PFGRX WT , ROGFP2 WT -PFGRX C88S , and ROGFP2 WT -PFGRX C32S were PCR-amplified with Phusion DNA polymerase using the corresponding p416TEF plasmids as a template 35 and subcloned into the Kpn I and Avr II restriction sites of pET45b in strain XL1-Blue

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J Biol Chem 272(19):1274712753 Show more references Kaelin WG Jr, Pallas DC, DeCaprio JA et al (1991) Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product

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To view a copy of this licence, visit About this article Cite this article Ye, K., Lan, R., Chen, Z